Molecular Pull-Down Assay

Neorabio's molecular pull-down workflow integrates both target-to-drug and drug-to-target strategies to support diverse experimental designs. Purified target proteins or immobilized small molecules are incubated with compounds, natural product extracts, or complex proteomes, followed by affinity enrichment, elution, and high-resolution LC-MS/MS analysis to identify interacting partners. Optimized immobilization chemistries and appropriate controls are applied to minimize non-specific binding, with replicate analyses ensuring data robustness. Resulting spectra are interpreted using advanced bioinformatic tools to generate annotated candidate lists, confidence metrics, and structured reports detailing assay conditions and analytical rationale.