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Protein expression and purification
Protein expression and purification
Our protein expression and purification service provides efficient expression systems and purification strategies to help clients obtain high-quality target proteins. We employ various expression systems such as E. coli, yeast, insect cells, and mammalian cells, optimizing conditions according to the properties and requirements of each protein. For purification, we apply techniques including affinity chromatography, ion-exchange chromatography, and gel filtration. This service is widely applicable to structural biology, drug screening, antibody engineering, and functional studies, ensuring that clients receive target proteins with high purity and strong biological activity.
Workflow
Customer provides target protein gene sequence or template → Gene synthesis or vector construction → Transformation into host cells (E. coli / yeast / mammalian cells, etc.) → Optimization of protein expression conditions → Protein extraction and purification → Quality testing (SDS-PAGE / Western Blot, etc.) → Protein functional validation and delivery → After-sales technical support
Core Offerings
• Protein preparation for structural biology • Recombinant proteins for functional and mechanistic studies • Antigen protein expression for antibody preparation • Target proteins for drug screening • Engineered and mutant protein expression and validation • Enzymes and industrial proteins for applied research • Recombinant proteins for vaccine development
Technical advantages
Our protein expression platform offers flexible systems including bacterial, yeast, insect, and mammalian cells, enabling efficient expression of proteins ranging from simple peptides to complex multi-subunit proteins. Optimized expression vectors and codon usage ensure high yield and solubility, while advanced purification workflows deliver proteins with excellent purity and activity. We support a wide range of modifications—such as fusion tags, post-translational modifications, and site-directed mutagenesis—to meet diverse research and therapeutic needs. Comprehensive quality control with SDS-PAGE, Western blot, and mass spectrometry guarantees reliability. These advantages make our services well-suited for structural biology, functional research, antibody production, drug screening, and vaccine development.
Fast response · Customized protein expression solutions
Expert team · Molecular biology & protein chemistry · Personalized support
QC methods: SDS-PAGE · Western Blot · Activity assays
Advanced purification · High purity · High stability
Optimized expression · Higher yield · Enhanced activity
Gene synthesis · Vector construction · Efficient expression
Multiple platforms · E. coli · Yeast · Mammalian cells
Service Offerings
Based on different application scenarios, we offer three categories of services: standardized, customized, and value-added.
E. coli expression system
Efficient expression of simple & tagged fusion proteins
Mammalian expression system
For complex proteins · With natural modifications
Yeast expression system
For proteins with post-translational modification needs
Insect cell expression system
For complex & multi-subunit proteins
Cell-free expression system
Fast synthesis · Small-scale · High-purity proteins
Protein mutant expression
Structure–function studies · Protein optimization
Fusion tag protein expression
His-tag · GST-tag · Multiple tag expression services
Deliverables
Certificate of Analysis (COA) and gel images for protein products